Epic Of Gilgamesh Essay Immortality Example For Students

Epic Of Gilgamesh Essay Immortality â€Å"But then I pose the inquiry: what number men must bite the dust before we can truly have a free and valid and serene society?How long will it take?If we can get the soul, and the genuine importance of this experience, I accept that this country can be changed into a general public of affection, of equity, harmony, and fraternity where all men can truly be brothers.† - Reverend Dr. Martin Luther King, Jr. Since the start of early human progress, contrasts in races and societies have been a piece of society. Alongside these distinctions, there advanced a contempt against what was not considered â€Å" the norm† . For a long time, bias, particularly as prejudice, has started many abhor violations and wars. Over ages, individuals have contrived procedures to battle these shameful acts in the best manner conceivable, regardless of whether it be respectful or vicious methods of dissent. August Wilson’s Pulitzer Prize winning play, â€Å" The Piano†, is set in the mid 1930s when prejudice was spreading like out of control fire. The play investigates two progressively various ways to deal with beating bias in America. Despite the fact that their methodologies contrast incredibly, both Berniece and Boy Willie both discover approaches to battle the issues related with living in a bigot culture. Servitude is still new in the psyches of numerous blacks and whites during the ‘30s as are numerous cruel sentiments. Berniece and Boy Willie tackle the prejudice of their time similarly their folks did. Bernice’s character is fundamentally the same as her mother’s, Mama Ola. She decides to maintain a strategic distance from clashes over prejudice at whatever point conceivable, regardless of whether it implies staying silent about subjects that ought to be tended to. She thinks that its simpler to â€Å" lay low† than to make a circumstance. Berniece sees the historical backdrop of the piano with a similar hatred and distress that her mom held for such a large number of years. In one of many warmed contentions with Boy Willie, Berniece says, â€Å" Mama Ola cleaned over this piano with her tears for a long time seventeen years worth of cold evenings and a vacant bed. For what a piano? To settle the score with someone. furthermore, what did it ever prompt? additional executing and more thieving.† When Boy Willie talks, one can nearly hear the life and assurance of his dad, Papa Boy Charles’ voice. He, much like his dad, has faith in the hypothesis: â€Å"by whatever implies necessary.† Boy Willie is eager to take the necessary steps and expel whoever holds him up; and that incorporates disposing of any white man that represents a danger against his fantasies. Kid Willie is glad that his dad gave his life to take the piano, with the carvings of his family’s history, from Sutter, the man who oppressed his distant grandma and his granddad. Daddy Boy Charles accepted that his family would consistently be slaves as long as Sutter still had responsibility for piano. Kid Willie discloses to Berniece that she should reveal to her little girl, Maretha, about the story behind the piano with the goal that she can be pleased with her granddad. â€Å"You should write down on the schedule the day that Papa Boy Charles carried that piano into the house. ..set up a gathering.. .have a celebration.† Although their perspectives are like their folks, they are extremely contradicted in their techniques for managing bigotry. When bigotry is at its top because of uncertain issues on the two sides, the future for blacks in America appears to be grim. In spite of the fact that subjection has finished, merciless assaults against blacks despite everything exist and many are more regrettable off monetarily than they were as slaves. Berniece takes a gander at her way of life from a realist’s perspective with little positive thinking. She sees no possibility of development for blacks and communicates this when she says, â€Å" I’m going to come clean with her.. .u726b64682c5961b4c973656a6f847114 , .u726b64682c5961b4c973656a6f847114 .postImageUrl , .u726b64682c5961b4c973656a6f847114 .focused content region { min-stature: 80px; position: relative; } .u726b64682c5961b4c973656a6f847114 , .u726b64682c5961b4c973656a6f847114:hover , .u726b64682c5961b4c973656a6f847114:visited , .u726b64682c5961b4c973656a6f847114:active { border:0!important; } .u726b64682c5961b4c973656a6f847114 .clearfix:after { content: ; show: table; clear: both; } .u726b64682c5961b4c973656a6f847114 { show: square; progress: foundation shading 250ms; webkit-change: foundation shading 250ms; width: 100%; darkness: 1; change: haziness 250ms; webkit-change: obscurity 250ms; foundation shading: #95A5A6; } .u726b64682c5961b4c973656a6f847114:active , .u726b64682c5961b4c973656a6f847114:hover { mistiness: 1; progress: murkiness 250ms; webkit-progress: obscurity 250ms; foundation shading: #2C3E50; } .u726b64682c5961b4c973656a6f847114 .focused content zone { width: 100%; position: relative; } .u726b64682c5961b4c973656a6f847114 .ctaText { fringe base: 0 strong #fff; shading: #2980B9; text dimension: 16px; textual style weight: intense; edge: 0; cushioning: 0; content enrichment: underline; } .u726b64682c5961b4c973656a6f847114 .postTitle { shading: #FFFFFF; text dimension: 16px; text style weight: 600; edge: 0; cushioning: 0; width: 100%; } .u726b64682c5961b4c973656a6f847114 .ctaButton { foundation shading: #7F8C8D!important; shading: #2980B9; outskirt: none; outskirt sweep: 3px; box-shadow: none; text dimension: 14px; textual style weight: striking; line-tallness: 26px; moz-fringe span: 3px; content adjust: focus; content enhancement: none; content shadow: none; width: 80px; min-tallness: 80px; foundation: url(https://artscolumbia.org/wp-content/modules/intelly-related-posts/resources/pictures/straightforward arrow.png)no-rehash; position: outright; right: 0; top: 0; } .u726b64682c5961b4c973656a6f847114:hover .ctaButton { foundation shading: #34495E!important; } .u726b 64682c5961b4c973656a6f847114 .focused content { show: table; stature: 80px; cushioning left: 18px; top: 0; } .u726b64682c5961b4c973656a6f847114-content { show: table-cell; edge: 0; cushioning: 0; cushioning right: 108px; position: relative; vertical-adjust: center; width: 100%; } .u726b64682c5961b4c973656a6f847114:after { content: ; show: square; clear: both; } READ: 12 Angry Men Essay.you at the base with all of us that’s exactly where she living.† Berneice accepts that blacks are at the base of life and they may never conquer their circumstance. In spite of the fact that she accepts that blacks can discover achievement; she feels that successs is restricted to the limits in which blacks are conceived. She follows the possibility that a few blacks allude to as â€Å" the house negro mentality†. This epithet was authored for those slaves who were OK with their ways of life since they don't saw anything great that could emerge out of opportunity. Berniece accepts blacks should thankfully take what is given to them and work with what they get. On the off chance that they become to ravenous they may end up with nothing. Shockingly, Boy Willie decides to be hopeful notwithstanding his environmental factors. In a world that reveals to him that there is no future for minorities, Boy Willie is a dreamer. He sees a future for himself past the shade of his skin and is resolved to go out and get it. He likewise realizes that he can't trust that white men will drop accomplishment on his lap; he needs to get it himself. In spite of his sister’s feelings, Boy Willie doesn't see himself at the base however rather at the highest point of society. â€Å"I’m living at the highest point of life. I ain’t going to simply end my life and discard it at the bottomI’m on the planet like every other person. † He further anxieties this supposition when he says, â€Å" how an individual perspectives himself is the means by which he will live†. Kid Willie reveals to Berniece that if an individual decides to accept he is at the base and there is no future; that is the means by which he will live at the base with no future.He additionally accepts that he is the equivalent and merits the same amount of as any white man. He considers himself to be an equivalent and maybe, happier than some white men. Kid Willie won't accept that white men are by one way or another superior to him or merit more than him. Kid Willie and Berneice unquestionably contrast by they way they handle supremacist circumstances and the treacheries submitted against them. Berniece decides to fight bigotry in an increasingly agreeable manner. She sees just the agony and grief that can come about because of standing firm against partiality. Her dad passed on attempting to demonstrate his masculinity to white men and she sees no good thing that can emerge out of standing up. Standing up can just make the day to day environment for blacks more regrettable in Berneice’s assessment. Kid Willie adopts a progressively aggressor strategy to doing combating prejudice. Kid Willie is intense and straightforward and couldn't care less whose feet he steps on to express what is on his mind. He is brave and maybe stupid when he says, â€Å" a nigger that ain’t hesitant to kick the bucket is the most noticeably terrible sort of nigger for the white man†. Kid Willie is eager to battle, or even slaughter, any white man that remains among him and opportunity. He discloses to Wining Boy that no white man would ever abuse him since he won't be abused. He treats individuals the manner in which they treat him. No more peculiar to prisons, Boy Willie shows total dismissal for the traditions that must be adhered to. He feels that the law possibly concerns him when it is helpful and fulfills his needs. He feels that the law was not intended for him however against him, and except if it accommodates his motivations he won't submit to it. Kid Willie won't permit anybody to administer over him in any way, regardless of whether that man is dark or white. In the play, â€Å" The Piano Lesson†, August Wilson’s characters offer two well known ways utilized by blacks for making due in a supremacist culture. In one case, the character of Berniece decide to live peacefully so as to maintain a strategic distance from any repurcussions that would come about because of standing up. Kid Willie decides to battle any man that means in his manner and attempts to keep him from finding the oppurtunity and opportunity that he merits. Albeit so

Free Essays on Lloyd George

What were the points of David Lloyd George’s social changes in the Edwardian time frame? England in the late nineteenth century was a world driving financial and military force. In spite of the fact that it was turning out to be progressively obvious that it was not going to stay a main politically influential nation, if the enormous social issues confronting a great part of the nation were not tended to. Issues, for example, destitution, joblessness, medicinal services and training. Seebohm Rowntree had featured the degree and reason for these social issues through his broad examinations and distribution of, Poverty, an investigation of Town Life in 1901. â€Å"Throughout the eighties and nineties, at an expanding rate, the nation’s attention to what was exhaustively called ‘the social problem’ had developed in width and depth.† It was very clear when Lloyd George was elevated to Chancellor of the Exchequer in 1906 that there was a requirement for new needs in the Liberals arrangements and he accepted that they lay in social changes. â€Å"He was chiefly looking for all gathering help for a program of household social change to handle lodging, medical coverage, joblessness, instruction, the poor law, liquor misuse and agriculture.† . Lloyd George realized that all together for the Liberal party to proceed in its prominence it required the help of the common laborers. It was the common laborers who might profit straightforwardly from social changes thus achieve support for the gathering and for Lloyd George. The huge requirement for social change was additionally filled by the way that Britain was as a mechanical force falling behind other world powers, for example, Germany and the United States. â€Å"Some businesses became mindful that the productivity of the workforce could be expanded not just by capital investment†¦but likewise by improving the effectiveness of human capital, for example by Improving working conditions and giving government assistance advantages to workers†¦Ã¢â‚¬  . It was the ideal opportunity for the legislature to step in and endeavor to pull Br... Free Essays on Lloyd George Free Essays on Lloyd George What were the points of David Lloyd George’s social changes in the Edwardian time frame? England in the late nineteenth century was a world driving monetary and military force. In spite of the fact that it was turning out to be progressively evident that it was not going to stay a main force to be reckoned with, if the huge social issues confronting a significant part of the nation were not tended to. Issues, for example, neediness, joblessness, human services and instruction. Seebohm Rowntree had featured the degree and reason for these social issues through his broad examinations and distribution of, Poverty, an investigation of Town Life in 1901. â€Å"Throughout the eighties and nineties, at an expanding rate, the nation’s attention to what was completely called ‘the social problem’ had developed in width and depth.† It was very clear when Lloyd George was elevated to Chancellor of the Exchequer in 1906 that there was a requirement for new needs in the Liberals strategies and he accepted that they lay in social changes. â€Å"He was essentially looking for all gathering help for a program of household social change to handle lodging, medical coverage, joblessness, instruction, the poor law, liquor misuse and agriculture.† . Lloyd George realized that all together for the Liberal party to proceed in its fame it required the help of the common laborers. It was the average workers who might profit straightforwardly from social changes thus achieve support for the gathering and for Lloyd George. The enormous requirement for social change was additionally filled by the way that Britain was as a modern force falling behind other world powers, for example, Germany and the United States. â€Å"Some managers became mindful that the proficiency of the workforce could be expanded not just by capital investment†¦but additionally by improving the effectiveness of human capital, for example by Improving working conditions and giving government assistance advantages to workers†¦Ã¢â‚¬  . It was the ideal opportunity for the legislature to step in and endeavor to pull Br...

Need for Professional Custom Essay Writing Service

Need for Professional Custom Essay Writing ServiceProfessional custom essay writing service is the best option for a student who wants to complete an essay that will make a difference in his or her life. Writing a good essay means writing an essay that is more relevant than other essays. Writing an essay that will help you achieve your goals, and the success of all others will greatly depend on how you write your essay.Essay writing services are able to write essays based on each student's needs. When you hire professional essay writing service, you will get a personalized service that will suit your needs. Whether you are just writing for your school or for an exam, these services provide the best solutions.The essence of writing an essay is to express your thoughts and ideas clearly and convincingly. There are so many things to consider when it comes to choosing the right essay topic. Some students feel too pressured to write their essay, while others are so busy with the daily lif e, and have to work a lot on their studies, they do not have enough time to write. In any case, for any student, writing an essay is a great way to meet your academic requirements.Most of the professional custom essay services offer their clients online writing service. Therefore, a customer can pay his or her dues online without dealing with delivery and post-purchase transactions. So, even if you are currently a student, you can still get the services of a professional custom essay writer.You can submit your essay as early as possible. But if you are not very sure of your essay, and need some tips, you can also start by reading some sample essays. See how the writer had completed his or her work, and what he or she liked about the article. By looking at the writing samples, you will be able to find the style of writing that will make you unique. If you are not sure what to write, you can simply look through these samples to see what's in common.To help you choose the right essay, you can ask the writer for a few suggestions. You can also take a few test essays and use the same approach to check out the format, the flow of words, the flow of the content, and the overall style of the essay. Once you are sure that you are going to use the same style of writing, it will be easier for you to choose the topic and write the essay.Many students find it difficult to complete their essay because they always have more to say than they actually do. Professional custom essay writing service has trained professionals who have done a great job to write essays. However, do not forget to put some time to review the written samples to get an idea of the style of writing.

The Universal Declaration Of Human Rights - 1417 Words

Implemented in 1948, the Universal Declaration of Human Rights (UDHR) delineates the basic rights and freedoms entitled to all humans. The freedom of speech and the right to express beliefs freely is a universal human right protected by Article 19 of the UDHR. It declares that â€Å"everyone has the right to freedom of opinion and expression† and can â€Å"hold opinions without interference†. The regional agreements of Iran, China and Bahrain are in accordance with the Universal Declaration and are fully dedicated to upholding the rights of the citizens, the government, and the overall welfare of their nation. While the government is often dealt a difficult hand with defining the boundaries of censorship, the idea of censorship is impermissible. The line between restricting and not restricting opinions becomes unclear when the universal right to free speech coincides with the basic rights of other citizens or openly threatens the balance of the state. Opinions contrary to that of the government do not justify refusing citizens their basic rights, like in the case of Iran, China and Bahrain. It is essential to protect free speech as a universal law for the benefit of society as a whole. The government of Iran, China, and Bahrain clearly violated the fundamental rights listed under the international human rights law. Therefore, they should be scrutinized for refusing citizens the ability to express their opinions. The following paragraphs will consider different cases that are inShow MoreRelatedThe Universal Declaration Of Human Rights1728 Words   |  7 Pagespeople. Culture can impede progress and leave women, minorities and other sub-sects of a society without the basic human rights that they deserve. Clinging too close to culture can be dangerous. The Foundations of a Universal Declaration The Universal Declaration of Human Rights (UDHR) was drafted shortly after the United Nations was established in 1945. The aim of the Declaration was to ensure that an atrocity such as the mass killings of Jews and other minorities in Nazi Germany would never happenRead MoreThe Universal Declaration of Human Rights1131 Words   |  5 PagesHuman rights are moral principles that set out specific standards of human behavior, and are normally ensured as lawful rights in both national and global law. They are acknowledged to be inalienable, since anybody is characteristically qualified for it essentially on the grounds that they are individuals. Whatever our nationality, sex, shade, religion, dialect, or ethnic source is, we are all just as qualified for our rights without separation or discrimination. All human rights are resolute andRead MoreThe Universal Declaration Of Human Rights Essay1368 Words   |  6 Pages The Universal Declaration of Human rights was adopted in the UN gene ral assembly by the 10th December 1948. This is the first time that the world recognized that everyone had the right to enjoy freedom of speech, freedom of religion and freedom from fear and want, and many other rights. International human rights come along way; before there was no rights. The idea of having rights that led to the development of international human rights takes time. There are benchmarks developments in internationalRead MoreThe Universal Declaration Of Human Rights Essay1276 Words   |  6 PagesA human right is an ethical choice and moral belief belonging to all humans regardless of traits, status, location, color, gender, or belief system. Making the connection to a universal law, the United Nations Rights High Commissioner explains these rights are, â€Å"guaranteed by law† and protected as â€Å"fundamental freedoms† (OHCHR, 2016). The Cambridge Dictionary defines privilege as â€Å"an advantage that only one person or group of people has† listing examples such as having a high social position or wealthRead MoreUniversal Declaration Of Human Rights1263 Words   |  6 PagesAccording to the Universal Declaration of Human Rights, a doctrine created to ensure a mutual standard of treatment amongst all humans, every person deserves an equal set of life standards. According to Article 18 of this 30 Article document, â€Å"everyone has the right to freedom of thought, conscience and religion; this right includes freedom to change his religion or belief and freedom, either alone or in community with others and in public or private, to manifest his religion or belief in teachingRead MoreThe Universal Declaration Of Human Rights892 Words   |  4 PagesHuman rigths is an essential component of a tolerant and individually satisfied society. They are created to defend people’s dignity, equality and liberty. However, for thousands of years people lived with no garanteed rights, until 1948, when United Nations adopted the Universal Declaration of Human Rights. But is the Universal Declaration of Human Rigths really universal to all states and humans living in them? I am going to argue if Human Rights should or should not be unically adapted to differentRead MoreThe Universal Declaration Of Human Rights875 Words   |  4 PagesI feel that the Universal Declaration of Human Rights (UDHR) does have a western bias. Many people look to US for guidance, but not many other nations copy our way of life. States can sign treaties, but they cannot be reassured that the other nation will keep its word. The US and the UN should maybe not be engaging in promoting western society, but they should be engaging in promoting the protection of human rights. The UN UDHR fought for minimal rights in 1948 by identifying three types of generationsRead MoreThe Universal Declaration Of Human Rights1485 Words   |  6 Pages1003236982 The Universal Declaration of Human Rights states â€Å"that human rights are held by all persons equally and universally forever† –hence, they are universal held. This is due to them being the exact same for all human beings anywhere in the world. One cannot acquire human rights because of where they come from, but because they are a member of the human race. Nobody can lose those human rights, nor can they be taken away for whatever the reason may be. Together, we have the right to express ourselvesRead MoreThe Declaration Of Universal Human Rights869 Words   |  4 PagesGeneral Assembly (UNGA) set forth a declaration of universal human rights. The goal was to set a common standard of rights based on â€Å"recognition of the inherent dignity and equal and inalienable rights of all members of the human family.† It was meant to become the perfect social contract but unfortunately was not upheld even by the signatory nations themselves. Many critics now looking b ack have cited the overreaching ideals as the downfall of the declaration but yet many have responded saying itRead MoreThe Universal Declaration Of Human Rights3323 Words   |  14 Pagesthe Universal Declaration of Human Rights (1948), the concept of human rights has gradually become one of the most commonly accepted universal norms, referred to in United Nations resolutions, national constitutions and regional and international treaties. Even so, human rights violations occur on an almost daily basis in countries around the world. The term seems to be at the forefront of contemporary political discourses, with its meaning at most times remaining unclear. In theory, human rights

Analysis Of Descartes s Meditation I - 989 Words

In Meditation I, Descartes discusses those things that can be called into doubt. He starts off by talking about how he has been mislead by his beliefs before, so will start off by separating those beliefs that cause him to doubt. He would like to secure his foundational beliefs similar to a house, without a secure foundation, the house can fall. Descartes mentions how he occasionally realizes that he has been deceived by his senses. Some things seem small at a distance, but then when looking at the same object, at a closer distance, it is not what you thought it was, your vision had deceived you; so he says it is better that you don’t trust those who may have even deceived you once, the senses. We should not depend on just the senses to make judgments, we should be aware of the fact that our senses can mislead us. He [Descartes] asks if he even exists, writing and sitting by the fire, if these hands are even his, but then he says these questions would lead him to be perceived as he is crazy, and he so ignores the idea. Next, in Meditation I, Descartes is wondering how he can tell if he is dreaming or awake. He imagines, what if he is not really sitting by the fire, in his gown, and writing, what if he is actually in bed and dreaming. When focusing and feeling on his hands, he realizes that he isn’t asleep, but there have been many times when he has assumed he is awake, when really he was dreaming. So he concludes that there is no way of really telling whetherShow MoreRelatedDescartes’ Cogito Argument Successfully Shows the Evil Demon Argument is Unsound888 Words   |  4 PagesDoes Descartes’ Cogito argument successfully show that the Evil Demon Argument is unsound? 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He is able to establish his own existence, but struggles to move beyondRead MoreThe Dream Inside Of A Dream By Christopher Nolan1683 Words   |  7 PagesPaper: Descartes The possibility of having a dream inside of a dream is an idea that has been discussed far and wide. However, before Inception came out in 2010 by director Christopher Nolan, many people in the modern world may not have ever considered this idea. Nonetheless, this idea of â€Å"a dream inside a dream† has been around since 1640, when Rene Descartes published Meditations. In Inception, Christopher Nolan uses Descartes ideas to enhance the storyline of his film. In this paper I willRead MoreAnalysis Of Descartes Meditations On First Philosophy Essay1758 Words   |  8 PagesRole of God in Descartes’ Meditations on First Philosophy In his work Meditations on First Philosophy, published in 1641, Renà © Descartes sets out to establish a set of indubitable truths for the sciences. He begins by discarding all of his beliefs, then works to rebuild his beliefs based on careful thought. Descartes clearly states this goal, saying in the First Meditation, â€Å"I will work my way up†¦ I will accomplish this by putting aside everything that admits of the least doubt† (I, 17). He is ableRead MoreDescartes Vs. Descartes s Theory Of Certainty1852 Words   |  8 Pagesof the greatest philosophers in history, Renà © Descartes tackles the daunting task of ensuring what is truly certain in life using a systemic thinking process known as methodological doubt in his Meditations on First Philosophy. In order to determine what is absolutely certain, Descartes concludes that the foundation which even reality sits upon is not firm since most of what contributed to its certainty is based on sensory perception. However, Descartes proves that even the senses can be deceivingRead MoreEssay on Descartes Failure4852 Words   |  20 PagesDescartes Failure In his Meditations on First Philosophy, Descartes strives first and foremost to provide an infallibly justified foundation for the empirical sciences, and second to prove the existence of God. I will focus on the first and second meditations in my attempt to show that, in his skepticism of the sources of knowledge, he fails to follow the rules he has set out in the Discourse on Method. First I claim that Descartes fails to draw the distinction between pure sensation andRead MoreDescartes Proof for the Existence of God Essay3414 Words   |  14 PagesDescartes Proof for the Existence of God Many readers follow Descartes with fascination and pleasure as he descends into the pit of skepticism in the first two Meditations, defeats the skeptics by finding the a version of the cogito, his nature, and that of bodies, only to find them selves baffled and repulsed when they come to his proof for the existence of God in Meditation III. In large measure this change of attitude results from a number of factors. One is that the proof is complicatedRead MoreEssay on The Philosophy of Cognitive Science2158 Words   |  9 Pagesproperly philosophical or require a scientific approach. First, I will expound the philosophical solution to the MBP proposed by Descartes, to be followed by an exposition of Ryles criticisms to the solution. Second, from Ryles criticism, I will deduce a scientific solution to the MBP related to the neural framework model of mind in cognitive science by means of what I call the principle of the embodiment of the mind. Finally, I shall point out the philosophi cal difficulties that are to be foundRead MoreA Brief Biography of Rene Descartes1580 Words   |  6 Pagesof philosopher Rene Descartes, who had endorsed the mechanistic conception of the world and the human body by coining notions such as mind, substance, and the knowledge argument; although his notions were not always accepted, he managed to provide valid support. Born in France, Rene Descartes, dubbed The Father of Modern Philosophy was, in a way, a Renaissance man having contributed influence amongst subjects such science, mathematics, psychology, and philosophy. Descartes was the medium of theRead MoreProposed Seven Philosophers On The Existence Of God And Their Development Of These Ideas1413 Words   |  6 Pages In my Round Table Essay I would like to introduce seven philosophers that we have discussed in class and focus on three specifically for my choice topic. The seven philosophers are as follows: (1) Socrates, (2) Plato, (3) Aristotle, (4) Francis Bacon, (5) St. Augustine, (6) Thomas Aquinas, and (7) Rene DesCartes. The specific three I want to focus on being; St. Augustine, Thomas Aquinas and Rene Descartes. Lastly, I will proceed to relate their ideas on the existence of God and their development

Cell differentiation by mechanical stress

Questions: 1. Compare and contrast primary cells and cell lines. In your answer, make sure to address the following points? 2. Describe how cell lines are maintained in the lab. In your answer, make sure to address the following points? 3. Describe the process of differentiation. In your answer make sure to address the following points? Answers: 1. a. The preparation of primary cells for cell culture. i. The organ is isolated and selected (Stacey, Doyle, Alan, Ferro, Margherita, 2002).ii. The organ is dissected to remove fat and necrotic cellsiii. The cells are subjected to enzymatic degradation (Stacey et al., 2002).iv. Trypsin or collagenase is addedv. Re-suspend and seed the medium Conditions needed for cell to survive include; gas mixture and temperature of 5% CO2 and 37 degrees Celsius respectively, appropriate PH, glucose and nutrients (Stacey et al., 2002). Advantages of primary cells over in vivo i. Use of primary cell is cost effective ("Primary Cell Culture | Sigma-Aldrich," n.d.). ii. Primary cells use avoids ethical objections. Disadvantages of primary cells i. Primary cells take longer to grow ii. Culture and isolations costs for primary cells are prohibitive and high c. Cell lines originate from a cell culture made from a single cell. The main distinguishing features of cell lines from primary cells include, cell lines have minimal variability while primary cells have variability existing between preparations and the donor cells ("Primary Cell Culture | Sigma-Aldrich," n.d.). d. Advantages of using cell lines over primary cells in experimentation i. Cell lines take less time to grow unlike primary cells ii. Cell lines have minimal variability unlike in primary cells. Disadvantages of using cell line over primary cells in experimentations i. They provide less relevant results as compared to primary cells ("Primary Cell Culture | Sigma-Aldrich," n.d.). 2. i. Confluence is the approximate number of the adherent cells in the flask or dish of the cell culture (Mather, 2008). ii. The procedure of cell line passaging starts with the removal of the medium of the HEK 293 cell flask completely (Masters, Stacey, 2007). This is then followed by addition of 3ml trypsin to the medium. The next step is placing the flask containing the medium to an incubator at a temperature of 37 degrees Celsius for 5 minutes (Masters, Stacey, 2007). Then rock the flask and wash down the cells by addition of medium and pipette to separate cell clusters into single cell lines. Add desirable volume of re-suspended cell mix into new plates. Finally, shake the plates or the flask in gentle manner to allow proper mixing then place them back to CO2 incubator at temperature of 37 degrees Celsius (Masters, Stacey, 2007).Passing cell lines helps to evade senescence. Passaging a cell line too many times results in cells having little in common with original reference strain. Preparation of cell lines for cryopreservation i. Harvesting cells in the log growth phase (Stacey, Hawkins, Fleck, 2011).ii. Bring both the semi-adherent and adherent cells into suspension by the use of EDTAiii. Remove a small aliquot of the cells and do cell quantification iv. Perform centrifugation on the remaining culture for approximately 5 minutesv. Re-suspend the cells in the freeze medium vi. Then pipette one aliquot of the cells vii. Place the ampoules in a freezer whose rate is controlled viii. The medium is then shifted to a freezer for cryopreservation 3. i. Differentiation is a process that happens in multicellular organisms where a cell with less specialization matures so that it becomes more discrete in its functions and form (Altman, 2001). The major importance of differentiation in multicellular organisms is that it provides them with a variety of cell specialized in performing specific functions. ii. Somatic cells differentiation starts with the chromatin for both plants and animals. Chromatin reorganization is the first steps that occurs which in turn lead to activation of the pluripotent genes including Oct3/4 in animals and NAM in plants, establishing pluripotentiality (Nakagawa, M., Yamanaka, 2010). There are a variety of activities that lead to chromatin modelling induced pluripotentiality that may include activity of the thrombin in serum, cell wall removal and viral infections. Basically, the presence of these stimuli signals the cells to differentiate thus that are how the cells know when to differentiate. iii. Stem cells have the following characteristics: Ability to divide and renew themselves Basic and unspecialized cells (Miki et al., 2005). Give rise to specialized cells through differentiation (Mather, 2008). According to Baker (2008) stem cells are appealing to the medical community because of their therapeutic potential. References Altman,G.H. (2001). Cell differentiation by mechanical stress. The FASEB Journal. doi:10.1096/fj.01-0656fje Baker,M. (2008). Cancer and stem cells: Beckman conference. Nature Reports Stem Cells. doi:10.1038/stemcells.2008.47 Masters,J.R., Stacey,G.N. (2007). Changing medium and passaging cell lines. Nature Protocols, 2(9), 2276-2284. doi:10.1038/nprot.2007.319 Mather,J.P. (2008). Stem cell culture. London: Elsevier/Academic Press. Miki,T., Lehmann,T., Cai,H., Stolz,D.B., Strom,S.C. (2005). Stem Cell Characteristics of Amniotic Epithelial Cells. Stem Cells, 23(10), 1549-1559. doi:10.1634/stemcells.2004-0357 Nakagawa,M., Yamanaka,S. (2010). Reprogramming of Somatic Cells to Pluripotency. Advances in Experimental Medicine and Biology, 215-224. doi:10.1007/978-1-4419-7037-4_14 Primary Cell Culture | Sigma-Aldrich. (n.d.). Retrieved from https://www.sigmaaldrich.com/technical-documents/articles/biology/primary-cell-culture.html Stacey,G., Doyle, Alan, Ferro, Margherita. (2002). Cell Culture Methods for in Vitro Toxicology. Springer Verlag. Stacey,G.N., Hawkins,R., Fleck,R.A. (2011). Cryopreservation and Banking of Cell Lines. Animal Cell Culture, 185-203. doi:10.1002/9780470669815.ch6

Vinegar Tom use of language Essay Example For Students

Vinegar Tom use of language Essay He doesnt show this from the beginning but its latter on in the play that you see his true colours; when Margery is taking a while to churn the butter. Jack enters and calls her a lazy slut, this shows his contempt for his wife and his short temper. Further evidence of this comes when he asks Alice to have an affair with him. The symbol of the request is in the apple but Jack changes his way of speaking to that of sympathy, not for anyone else but himself. He tries to gain the sympathy of Alice by acting hard done by as if in need of her. We will write a custom essay on Vinegar Tom use of language specifically for you for only $16.38 $13.9/page Order now When talking of his wife he says, Im no good to my wife. I cant do it. Not these last three months. Its only when I dream of you or like now talking to you he then pleads with Alice to have some pity. When Alice doesnt fall for his act he takes it out on Joan calling her an ungrateful old hag, and telling her hell break your neck if you speak to me. From this point on his is back to his miserable and aggressive mood which he keeps until the end of the play when he gets Joan hung, losing it only for a short time when Alice gives him it back. Joan Joan plays the act of an old sweet woman trying to get attention, yet when she is provoked she can be as spiteful and wicked as a witch and has a foul tongue. Examples of these two sides to her personality can be seen when she visits Margery in the barn in scene five and asks for some yeast. At first she is as Jack is in scene five with Alice trying to get sympathy A small crumb of yeast and God will bless you for your kindness to your poor old neighbour. Yet when she receives none and Margery is a little rude to her she responds by cursing her and saying; Devil take you and your man and your yeast and your beer and your bread and your cider and your cold face and she would have continued if she wasnt cut short by Margery. The way she starts of by talking of blessings from God to cursing from the devil shows the two sides of Joans personality, she is in fact a poor old woman, but she is so bitter by that that she curses and swears like a witch which is what gets her hanged in the end.